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KMID : 0861020130280060119
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Korea Journal of Herbology
2013 Volume.28 No. 6 p.119 ~ p.127
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Effects of β-Asarone on Pro-Inflammatory Cytokines and Learning and Memory Impairment in Lipopolysaccharide-Treated Mice
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Choi Moon-Sook
Kwak Hee-Jun
Kweon Ki-Jung
Hwang Ji-Mo
Shin Jung-Won
Sohn Nak-Won
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Abstract
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Objectives : β-Asarone (BAS) is an active ingredient in Acori Rhizoma. This study investigated anti-neuroinflammatory and memory ameliorating effects of BAS in systemic lipopolysaccharide (LPS)-treated C57BL/6 mice.
Methods : BAS was administered orally at doses of 7.5, 15, and 30 mg/kg for 3 days prior to LPS (3 mg/kg, intraperitoneal) injection. Pro-inflammatory cytokine mRNA, including tumor necrosis factor-α (TNF-ㅍ), interleukin (IL)-1β and IL-6, was measured in hippocampus tissue using real-time polymerase chain reaction at 4 h after the LPS injection. An ameliorating effect of 30 mg/kg BAS on learning and memory impairment in the LPS-treated mice was verified using the Morris water maze test.
Results : BAS significantly attenuated up-regulation of TNF-α, IL-1β, and IL-6 mRNA in hippocampus tissue of the LPS-treated mice. In acquisition training test, BAS improved learning performance of the LPS-treated mice with a significant decrease of escape latency to the platform. In memory retention test, BAS also ameliorated memory impairment of the LPS-treated mice with a significant increase of swimming time in zones neighboring to the platform, number of target heading, and memory score.
Conclusion : The results suggest that inhibition of pro-inflammatory cytokines and neuroinflammation in the hippocampus by BAS could be one of the mechanisms for BAS-mediated ameliorating effect on learning and memory impairment in LPS-treated mice.
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KEYWORD
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β-Asarone, Acori Rhizoma, neuroinflammation, memory impairment, Morris water maze
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